Price range: $268.00 through $328.00
Background:
This gene encodes a member of the epsin protein family. The encoded protein binds clathrin and is involved in the endocytosis of clathrin-coated vesicles. Loss of function of this gene is associated with reduced tumor growth and progression in certain cancer types. [provided by RefSeq, Mar 2016],
| Size | 50μL/100μL |
| Target | Epsin 1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 85kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:40000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from Epsin 1 . at AA range: 140-220 |
| Specificity | Epsin 1 Polyclonal Antibody detects endogenous levels of Epsin 1 protein. |
| Gene Name | EPN1 |
| Protein Name | Epsin-1 |
| Other Name | EPN1; / Epsin-1; / EH domain-binding mitotic phosphoprotein; / EPS-15-interacting protein 1 |
| Species | Human |
| Gene ID | 29924 |
| Uniprot | Q9Y6I3, |
| Species.1 | Mouse |
| Gene ID.1 | 13854 |
| Uniprot.1 | Q80VP1, |
| Species.2 | Rat |
| Gene ID.2 | 117277 |
| Uniprot.2 | O88339 |
| Cellular Localization | Cytoplasm . Cell membrane; Peripheral membrane protein . Nucleus . Membrane, clathrin-coated pit . Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity). . |
| Tissue Expression | Brain,Epithelium,Mammary gland, |
| Signaling_pathway | |
| Research Areas | >>Endocytosis |
| Function | Domain:The DPW repeat domain is involved in AP2A2 and clathrin binding.,Domain:The NPF repeat domain is involved in EPS15 binding.,Function:Binds to membranes enriched in phosphatidylinositol-4,5-biphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.,online information:The bubble's bend -Issue 42 of January 2004,PTM:Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.,PTM:Ubiquitinated.,similarity:Belongs to the epsin family.,similarity:Contains 1 ENTH (epsin N-terminal homology) domain.,similarity:Contains 2 UIM (ubiquitin-interacting motif) repeats.,subcellular location:Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145.,subunit:Monomer. Binds clathrin, ZBTB16/ZNF145 and ITSN1. Binds ubiquitinated proteins (By similarity). Binds REPS2, EPS15, AP2A1 and AP2A2. Interacts with RALBP1 in a complex also containing NUMB and TFAP2A during interphase and mitosis., |
