Price range: $268.00 through $328.00
Background:
This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Epsin 2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 68kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EPN2. AA range:271-320 |
| Specificity | Epsin 2 Polyclonal Antibody detects endogenous levels of Epsin 2 protein. |
| Gene Name | EPN2 |
| Protein Name | Epsin-2 |
| Other Name | EPN2; / KIAA1065; / Epsin-2; / EPS-15-interacting protein 2 |
| Species | Human |
| Gene ID | 22905 |
| Uniprot | O95208, |
| Species.1 | Mouse |
| Gene ID.1 | 13855 |
| Uniprot.1 | Q8CHU3, |
| Species.2 | Rat |
| Gene ID.2 | 60443 |
| Uniprot.2 | Q9Z1Z3 |
| Cellular Localization | Cytoplasm . Cytoplasmic vesicle, clathrin-coated vesicle . In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex. |
| Tissue Expression | Highest expression is found in brain. Detected at lower levels in lung and liver. |
| Signaling_pathway | |
| Research Areas | >>Endocytosis |
| Function | Caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,Domain:The DPW repeat domain is involved in AP-2 and clathrin binding.,Domain:The NPF repeat domain is involved in EPS15 binding.,Function:Plays a role in the formation of clathrin-coated invaginations and endocytosis.,PTM:Ubiquitinated.,similarity:Belongs to the epsin family.,similarity:Contains 1 ENTH (epsin N-terminal homology) domain.,similarity:Contains 2 UIM (ubiquitin-interacting motif) repeats.,subcellular location:In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex.,subunit:Binds EPS15 (By similarity). Binds AP-2 and clathrin.,tissue specificity:Highest expression is found in brain. Detected at lower levels in lung and liver., |
