ERp57 Polyclonal Antibody
Ucallm_WP15294-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | ERp57 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 55kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human PDIA3. AA range:111-160 |
| Specificity | ERp57 Polyclonal Antibody detects endogenous levels of ERp57 protein. |
| Gene Name | PDIA3 |
| Protein Name | Protein disulfide-isomerase A3 |
| Other Name | PDIA3; / ERP57; / ERP60; / GRP58; / Protein disulfide-isomerase A3; / 58 kDa glucose-regulated protein; / 58 kDa microsomal protein; / p58; / Disulfide isomerase ER-60; / Endoplasmic reticulum resident protein 57; / ER protein 57; / ERp57; / Endoplasmic reticulum resident protein 60; / ER protein 60; / ERp60 |
| Species | Human |
| Gene ID | 2923 |
| Uniprot | P30101, |
| Species.1 | Mouse |
| Gene ID.1 | 14827 |
| Uniprot.1 | P27773, |
| Species.2 | Rat |
| Gene ID.2 | 29468 |
| Uniprot.2 | P11598 |
| Cellular Localization | Endoplasmic reticulum . Endoplasmic reticulum lumen . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). . |
| Tissue Expression | Detected in the flagellum and head region of spermatozoa (at protein level) (PubMed:20400973). Expressed in liver, stomach and colon (at protein level). Expressed in gastric parietal cells and chief cells (at protein level) (PubMed:24188822). |
| Signaling_pathway | Organismal Systems >> Immune system >> Antigen processing and presentation;Genetic Information Processing >> Folding, sorting and degradation >> Protein processing in endoplasmic reticulum |
| Research Areas | >>Protein processing in endoplasmic reticulum; / >>Antigen processing and presentation; / >>Human cytomegalovirus infection; / >>Herpes simplex virus 1 infection; / >>Epstein-Barr virus infection; / >>Human immunodeficiency virus 1 infection |
| Function | Catalytic activity:Catalyzes the rearrangement of -S-S- bonds in proteins.,Caution:Was originally thought to be a phosphatidylinositol-4,5-bisphosphate phosphodiesterase type I (phospholipase C-alpha).,mass spectrometry: PubMed:11840567,similarity:Belongs to the protein disulfide isomerase family.,similarity:Contains 2 thioredoxin domains.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Interacts with ERP27 and CANX., |
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