Price range: $268.00 through $328.00
Background:
This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015],
| Size | 50μL/100μL |
| Target | ET-1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 25kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC: 100-300; WB 1:500-1:2000; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human EDN1. AA range:131-180 |
| Specificity | ET-1 Polyclonal Antibody detects endogenous levels of ET-1 protein. |
| Gene Name | EDN1 |
| Protein Name | Endothelin-1 |
| Other Name | EDN1; / Endothelin-1; / Preproendothelin-1; / PPET1 |
| Species | Human |
| Gene ID | 1906 |
| Uniprot | P05305, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P22387 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Secreted. |
| Tissue Expression | Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Human Diseases >> Cancer: overview >> Pathways in cancer;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway |
| Research Areas | >>cAMP signaling pathway; / >>HIF-1 signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>Vascular smooth muscle contraction; / >>TNF signaling pathway; / >>Melanogenesis; / >>Renin secretion; / >>Relaxin signaling pathway; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Pathways in cancer; / >>Hypertrophic cardiomyopathy; / >>Fluid shear stress and atherosclerosis |
| Function | Function:Endothelins are endothelium-derived vasoconstrictor peptides.,online information:Endothelin entry,similarity:Belongs to the endothelin/sarafotoxin family.,tissue specificity:Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells., |
