Price range: $268.00 through $328.00
Background:
Fascin is a highly conserved actin bundle protein, which is located in microfilaments and stress fibers involved in microfilament bundle formation. It is mainly expressed in dendritic cells and is a sensitive marker of R-S cells. Lymphocytes, plasma cells and myeloid cells did not express. It can be used to diagnose Hodgkin’s lymphoma and Langerhans cell histiocytosis.
| Size | 50μL/100μL |
| Target | Fascin |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 55kD |
| MW(Observed) | 55kD |
| Host Species | Mouse |
| Isotype | IgG1,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM-FASN |
| Immunogen | Synthesized peptide derived from human Fascin AA range: 250-350 |
| Specificity | The antibody can specifically recognize human Fascin protein. In western blotting of Hela cell lysate, the antibody can label a 55 kDa band corresponding to Fascin, while there is no band detected in Fascin knock out Hela cell lysate. |
| Gene Name | FSCN1 FAN1 HSN SNL |
| Protein Name | Fascin (55 kDa actin-bundling protein) (Singed-like protein) (p55) |
| Other Name | 55 kDa actin bundling protein; / 55 kDa actin-bundling protein; / Actin bundling protein; / actin bundling protein, 55-KD; / FAN 1; / FAN1; / Fascin 1; / Fascin actin bundling protein 1; / Fascin; / Fascin homolog 1 actin bundling protein; / Strongylocentrotus purpuRatus; / Fascin homolog 1; / Fascin, sea urchin, homolog of, 1; / Fascin1; / FLJ38511; / FSCN 1; / FSCN1; / FSCN1_HUMAN; / HSN; / p55; / Singed; / Drosophila; / like; / sea urchin fascin homolog like; / Singed drosophila homolog like; / Singed like; / fascin homolog sea urchin; / Drosophila; / Singed like; / fascin homolog sea urchin; / Singed like protein; / Singed, drosophila, homolog of; / Singed-like protein; / SNL; / Strongylocentrotus purpuRatus |
| Species | Human |
| Gene ID | 6624 |
| Uniprot | Q16658, |
| Species.1 | Mouse |
| Gene ID.1 | 14086 |
| Uniprot.1 | Q61553, |
| Species.2 | Rat |
| Gene ID.2 | 683788 |
| Uniprot.2 | P85845 |
| Cellular Localization | Cytoplasmic |
| Tissue Expression | Ubiquitous. |
| Signaling_pathway | Human Diseases >> Cancer: overview >> MicroRNAs in cancer |
| Research Areas | >>MicroRNAs in cancer |
| Function | Disease:Marks and mediates breast cancer metastasis to the lungs. FSCN1 is not functionally validated but achieves the highest statistical significance (P less than 0.000001). Those subjects expressing the lung metastasis signature have a significantly poorer lung metastasis-free survival, but not bone metastasis-free survival, compared to subjects without the signature.,Function:Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers.,PTM:Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin.,similarity:Belongs to the fascin family.,subunit:Associates with beta-catenin.,tissue specificity:Ubiquitous., |
