Price range: $268.00 through $328.00
Background:
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Fatty Acid Synthase |
| Reactivity | Human, Mouse, Rat, Monkey, Bovine |
| Applications | WB, IP, IF |
| MW(Calculated) | |
| MW(Observed) | 273kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000; ICC 1:400; IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 13G8 |
| Immunogen | Purified recombinant human Fatty Acid Synthase protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of Fatty Acid Synthase and does not cross-react with related proteins. |
| Gene Name | fasn |
| Protein Name | |
| Other Name | [Acyl-carrier-protein] S acetyltransferase; / [Acyl-carrier-protein] S malonyltransferase; / 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase; / 3-oxoacyl-[acyl-carrier-protein] reductase; / 3-oxoacyl-[acyl-carrier-protein] synthase; / Enoyl-[acyl-carrier-protein] |
| Species | Human |
| Gene ID | 2194 |
| Uniprot | P49327, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P19096 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Tissue Expression | Ubiquitous. Prominent expression in brain, lung, liver and mammary gland. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway |
| Research Areas | >>Fatty acid biosynthesis; / >>Metabolic pathways; / >>Fatty acid metabolism; / >>AMPK signaling pathway; / >>Insulin signaling pathway; / >>Alcoholic liver disease |
| Function | Catalytic activity:(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH.,Catalytic activity:(3R)-3-hydroxypalmitoyl-[acyl-carrier-protein] = hexadec-2-enoyl-[acyl-carrier-protein] + H(2)O.,Catalytic activity:Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein].,Catalytic activity:Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO(2) + 2n NADP(+).,Catalytic activity:Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein].,Catalytic activity:Acyl-[acyl-carrier-protein] + NADP(+) = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADPH.,Catalytic activity:Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein].,Catalytic activity:Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate.,Function:Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.,miscellaneous:The relatively low beta-ketoacyl synthase activity may be attributable to the low 4'-phosphopantetheine content of the protein.,sequence Caution:Several sequencing errors.,similarity:Contains 1 acyl carrier domain.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer which is arranged in a head to tail fashion.,tissue specificity:Ubiquitous. Prominent expression in brain, lung, and liver., |
