Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a member of the FPS/FES family of non-transmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome X. [provided by RefSeq, Apr 2015],
| Size | 50μL/100μL |
| Target | FER |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 95kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FER. AA range:766-815 |
| Specificity | Fer Polyclonal Antibody detects endogenous levels of Fer protein. |
| Gene Name | FER |
| Protein Name | Tyrosine-protein kinase Fer |
| Other Name | FER; / TYK3; / Tyrosine-protein kinase Fer; / Feline encephalitis virus-related kinase FER; / Fujinami poultry sarcoma/Feline sarcoma-related protein Fer; / Proto-oncogene c-Fer; / Tyrosine kinase 3; / p94-Fer |
| Species | Human |
| Gene ID | 2241 |
| Uniprot | P16591, |
| Species.1 | Mouse |
| Gene ID.1 | 14158 |
| Uniprot.1 | P70451, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P09760 |
| Cellular Localization | Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell junction. Membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Cytoplasm, cell cortex. Associated with the chromatin. Detected on microtubules in polarized and motile vascular endothelial cells. Colocalizes with F-actin at the cell cortex. Colocalizes with PECAM1 and CTNND1 at nascent cell-cell contacts. |
| Tissue Expression | Isoform 1 is detected in normal colon and in fibroblasts (at protein level). Isoform 3 is detected in normal testis, in colon carcinoma-derived metastases in lung, liver and ovary, and in colon carcinoma and hepato carcinoma cell lines (at protein level). Isoform 3 is not detected in normal colon or in normal fibroblasts (at protein level). Widely expressed. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Adherens junction |
| Research Areas | >>Adherens junction |
| Function | Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Function:Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.,similarity:Contains 1 FCH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,subcellular location:Associated with the chromatin.,tissue specificity:Expressed in a variety of lymphoid cell lines., |
