Name: U-Blot® FLT3 Rabbit mAb
SKU: WR4967
Price: 268 USD
Availability: InStock

Product details

Background:This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jan 2015],

Specifications

Size	50μL/100μL
Target	Flt3
Reactivity	Human, Mouse
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	113kD
MW(Observed)	160kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1191R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	FLT3
Protein Name	Receptor-type tyrosine-protein kinase FLT3
Other Name	FLT3; / CD135; / FLK2; / STK1; / Receptor-type tyrosine-protein kinase FLT3; / FL cytokine receptor; / Fetal liver kinase-2; / FLK-2; / Fms-like tyrosine kinase 3; / FLT-3; / Stem cell tyrosine kinase 1; / STK-1; / CD antigen CD135
Species	Human
Gene ID	2322
Uniprot	P36888,
Species.1	Mouse
Gene ID.1	14255
Uniprot.1	Q00342
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Membrane; Single-pass type I membrane protein. Endoplasmic reticulum lumen. Constitutively activated mutant forms with internal tandem duplications are less efficiently transported to the cell surface and a significant proportion is retained in an immature form in the endoplasmic reticulum lumen. The activated kinase is rapidly targeted for degradation.
Tissue Expression	Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells (at protein level). Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas. Highly expressed in T-cell leukemia.
Signaling_pathway	Organismal Systems >> Immune system >> Hematopoietic cell lineage;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Research Areas	>>MAPK signaling pathway; / >>Ras signaling pathway; / >>PI3K-Akt signaling pathway; / >>Hematopoietic cell lineage; / >>Pathways in cancer; / >>Transcriptional misregulation in cancer; / >>Acute myeloid leukemia; / >>Central carbon metabolism in cancer
Function	Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Function:Receptor for the FL cytokine. Has a tyrosine-protein kinase activity.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.,similarity:Contains 1 Ig-like C2-type (immunoglobulin-like) domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with FIZ1 following ligand activation.,tissue specificity:Bone marrow cells.,