Name: Gβ 5 Polyclonal Antibody
SKU: WP12640
Price: 268 USD
Availability: InStock

Product details

Background:

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Jul 2008],

Specifications

Target	GNB5
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	40kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human GNB5. AA range:151-200
Sequence
Specificity	Gβ 5 Polyclonal Antibody detects endogenous levels of Gβ 5 protein.
Gene Name	GNB5
Protein Name	Guanine nucleotide-binding protein subunit beta-5
Other Name	GNB5; / Guanine nucleotide-binding protein subunit beta-5; / Gbeta5; / Transducin beta chain 5
Species	Human
Gene ID-1	10681
Uniprot	O14775,
Species.1	Mouse
Gene ID-2	14697
Uniprot.1	P62881,
Species.2	Rat
Gene ID-3	83579
Uniprot.2	P62882
Organism-4
Gene ID-4
SwissProt-4
Background	Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Jul 2008],
Cellular Localization	Membrane .
Tissue Expression	Widely expressed.
Signaling_pathway	Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Human Diseases >> Cancer: overview >> Pathways in cancer;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Research Areas	>>Ras signaling pathway; / >>Chemokine signaling pathway; / >>PI3K-Akt signaling pathway; / >>Apelin signaling pathway; / >>Circadian entrainment; / >>Retrograde endocannabinoid signaling; / >>Glutamatergic synapse; / >>Cholinergic synapse; / >>Serotonergic synapse; / >>GABAergic synapse; / >>Dopaminergic synapse; / >>Relaxin signaling pathway; / >>Morphine addiction; / >>Alcoholism; / >>Human cytomegalovirus infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Human immunodeficiency virus 1 infection; / >>Pathways in cancer
Function	Function:Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.,similarity:Belongs to the WD repeat G protein beta family.,similarity:Contains 7 WD repeats.,subunit:G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1), Gbeta5 (GNB5) and RGS9BP.,tissue specificity:Expressed in multiple tissues.,
RRID