U-Blot® GCLM Rabbit mAb
WR4332 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4332 Western blot analysis of lysates from A549 cells, using MMP7 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015],
Specifications
| Size | 50μL/100μL |
| Target | GCLM |
| Reactivity | Human |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 31kD |
| MW(Observed) | 31kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0478R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | GCLM |
| Protein Name | Glutamate--cysteine ligase regulatory subunit |
| Other Name | GCLM; / GLCLR; / Glutamate--cysteine ligase regulatory subunit; / GCS light chain; / Gamma-ECS regulatory subunit; / Gamma-glutamylcysteine synthetase regulatory subunit; / Glutamate--cysteine ligase modifier subunit |
| Species | Human |
| Gene ID | 4316 |
| Uniprot | P48507, |
| Species.1 | Mouse |
| Gene ID.1 | 14630 |
| Uniprot.1 | O09172, |
| Species.2 | Rat |
| Gene ID.2 | 25335 |
| Uniprot.2 | P50280 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Colon,Human small intestine,Kidney,Placenta, |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Wnt signaling pathway |
| Research Areas | >>Wnt signaling pathway; / >>Human T-cell leukemia virus 1 infection |
| Function | pathway:Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.,similarity:Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.,subunit:Heterodimer of a catalytic heavy chain and a regulatory light chain.,tissue specificity:In all tissues examined. Highest levels in skeletal muscle., |
Please login to post questions
Related products
