Price range: $268.00 through $328.00
Background:
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008],
| Target | GFAP |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF |
| MW(Calculated) | |
| MW(Observed) | 45kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | WB 1:2000-5000; IF 1:200; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004) |
| Formulation | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Source | |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 5C8 |
| Immunogen | Synthetic Peptide of GFAP |
| Sequence | |
| Specificity | The antibody detects endogenous GFAP proteins. |
| Gene Name | GFAP |
| Protein Name | Glial fibrillary acidic protein |
| Other Name | GFAP; / Glial fibrillary acidic protein; / GFAP |
| Species | Human |
| Gene ID-1 | 2670 |
| Uniprot | P14136, |
| Species.1 | Mouse |
| Gene ID-2 | 14580 |
| Uniprot.1 | P03995, |
| Species.2 | Rat |
| Gene ID-3 | 24387 |
| Uniprot.2 | P47819 |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008], |
| Cellular Localization | Cytoplasm . Associated with intermediate filaments. . |
| Tissue Expression | Expressed in cells lacking fibronectin. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway |
| Research Areas | >>JAK-STAT signaling pathway |
| Function | Alternative products:Isoforms differ in the C-terminal region which is encoded by alternative exons,Disease:Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.,Function:GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.,online information:GFAP entry,similarity:Belongs to the intermediate filament family.,subcellular location:Associated with intermediate filaments.,subunit:Interacts with SYNM (By similarity). Isoform 3 interacts with PSEN1 (via N-terminus).,tissue specificity:Expressed in cells lacking fibronectin., |
| RRID |
