Name: GGT1 Polyclonal Antibody
SKU: WP15334
Price: 268 USD
Availability: InStock

Product details

Background:

The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014],

Specifications

Target	GGT1
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	61kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from the N-terminal region of human GGT1. AA range:21-70
Sequence
Specificity	GGT1 Polyclonal Antibody detects endogenous levels of GGT1 protein.
Gene Name	GGT1
Protein Name	Gamma-glutamyltranspeptidase 1
Other Name	GGT1; / GGT; / Gamma-glutamyltranspeptidase 1; / GGT 1; / Gamma-glutamyltransferase 1; / Glutathione hydrolase 1; / Leukotriene-C4 hydrolase; / CD224
Species	Human
Gene ID-1	2678
Uniprot	P19440,
Species.1	Mouse
Gene ID-2
Uniprot.1	Q60928
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014],
Cellular Localization	Cell membrane; Single-pass type II membrane protein .
Tissue Expression	Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein.; [Isoform 3]: Lung-specific.
Signaling_pathway
Research Areas	>>Taurine and hypotaurine metabolism; / >>Glutathione metabolism; / >>Arachidonic acid metabolism; / >>Metabolic pathways
Function	Catalytic activity:(5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl amino acid.,Disease:Defects in GGT1 are a cause of glutathionuria [MIM:231950]; also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease.,Function:Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracelular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive.,Function:Initiates extracellular glutathione (GSH) breakdown; catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors.,miscellaneous:Corresponds to the light chain of other gamma-glutamyltransferase family members.,miscellaneous:Cys-454 was thought to bind the gamma-glutamyl moiety, but mutagenesis of this residue had no effect on activity.,online information:Gamma-glutamyl transpeptidase entry,pathway:Sulfur metabolism; glutathione metabolism.,PTM:N-glycosylated on both chains. Contains hexoses, hexosamines and sialic acid residues. It is not known if the sialic acid residues are present on N-linked or on O-linked glycans.,similarity:Belongs to the gamma-glutamyltransferase family.,subunit:Heterodimer composed of the light and heavy chains. The active site is located in the light chain.,tissue specificity:Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Isoform 3 is lung-specific. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein.,tissue specificity:Highly expressed in fetal and adult kidney and liver.,
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GGT1 Polyclonal Antibody

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GGT1 Polyclonal Antibody

Product details

Specifications

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