Price range: $268.00 through $328.00
Background:
The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011],
| Size | 50μL/100μL |
| Target | GK1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 57kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GK. AA range:461-510 |
| Specificity | GK1 Polyclonal Antibody detects endogenous levels of GK1 protein. |
| Gene Name | GK |
| Protein Name | Glycerol kinase |
| Other Name | GK; / Glycerol kinase; / GK; / Glycerokinase; / ATP:glycerol 3-phosphotransferase |
| Species | Human |
| Gene ID | 2710 |
| Uniprot | P32189, |
| Species.1 | Mouse |
| Gene ID.1 | 14933 |
| Uniprot.1 | Q64516, |
| Species.2 | Rat |
| Gene ID.2 | 79223 |
| Uniprot.2 | Q63060 |
| Cellular Localization | Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm. |
| Tissue Expression | Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> PPAR signaling pathway |
| Research Areas | >>Glycerolipid metabolism; / >>Metabolic pathways; / >>PPAR signaling pathway |
| Function | Catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate.,Caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,Disease:Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.,Function:Key enzyme in the regulation of glycerol uptake and metabolism.,pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.,similarity:Belongs to the FGGY kinase family.,subcellular location:In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.,tissue specificity:Highly expressed in the liver, kidney and testis. Isoforms 2 and 3 are expressed specifically in testis and fetal liver, but not in the adult liver., |
