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Home/Reagents/Antibody/Primary antibody/Polyclonal Antibody/Rabbit pAb/Glucosidase IIα Polyclonal Antibody
SKU: WP12486 Categories: ,

Glucosidase IIα Polyclonal Antibody

Ucallm_WP12486-50

Price range: $268.00 through $328.00

SKU: WP12486-100
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Product details

Background:

This gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease. [provided by RefSeq, Jul 2016],

Specifications

TargetGlucosidase IIα
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)107kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Modification
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human GANAB. AA range:242-291
Sequence
SpecificityGlucosidase IIα Polyclonal Antibody detects endogenous levels of Glucosidase IIα protein.
Gene NameGANAB
Protein NameNeutral alpha-glucosidase AB
Other NameGANAB; / G2AN; / KIAA0088; / Neutral alpha-glucosidase AB; / Alpha-glucosidase 2; / Glucosidase II subunit alpha
SpeciesHuman
Gene ID-123193
UniprotQ14697,
Species.1Mouse
Gene ID-214376
Uniprot.1Q8BHN3
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
BackgroundThis gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease. [provided by RefSeq, Jul 2016],
Cellular LocalizationEndoplasmic reticulum . Golgi apparatus . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .
Tissue ExpressionDetected in placenta (PubMed:3881423). Isoform 1 and isoform 2 are expressed in the kidney and liver (PubMed:27259053).
Signaling_pathwayGenetic Information Processing >> Folding, sorting and degradation >> Protein processing in endoplasmic reticulum
Research Areas>>N-Glycan biosynthesis; / >>Metabolic pathways; / >>Protein processing in endoplasmic reticulum
FunctionCatalytic activity:Hydrolysis of terminal (1->3)-alpha-D-glucosidic links in (1->3)-alpha-D-glucans.,Function:Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins.,pathway:Glycan metabolism; N-glycan metabolism.,similarity:Belongs to the glycosyl hydrolase 31 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC.,tissue specificity:Detected in placenta.,
RRID
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