U-Blot® GluR-2 Rabbit mAb
Ucallm_WR4888-50
Price range: $268.00 through $328.00
Product details
Background:Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants enco
Specifications
| Size | 50μL/100μL |
| Target | GluR-2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 99kD |
| MW(Observed) | 99kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:10000-1:50000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1058R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | GRIA2 GLUR2 |
| Protein Name | Glutamate receptor 2 |
| Other Name | GRIA2; / GLUR2; / Glutamate receptor 2; / GluR-2; / AMPA-selective glutamate receptor 2; / GluR-B; / GluR-K2; / Glutamate receptor ionotropic; / AMPA 2; / GluA2 |
| Species | Human |
| Gene ID | 2891 |
| Uniprot | P42262, |
| Species.1 | Mouse |
| Gene ID.1 | 14800 |
| Uniprot.1 | P23819, |
| Species.2 | Rat |
| Gene ID.2 | 29627 |
| Uniprot.2 | P19491 |
| Cellular Localization | Cell membrane; Multi-pass membrane protein . Endoplasmic reticulum membrane; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein . Cell junction, synapse, postsynaptic density membrane; Multi-pass membrane protein . Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression (By similarity). Displays a somatodendritic localization and is excluded from axons in neurons (By similarity). . |
| Tissue Expression | Brain, |
| Signaling_pathway | Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway |
| Research Areas | >>cAMP signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>Circadian entrainment; / >>Long-term potentiation; / >>Retrograde endocannabinoid signaling; / >>Glutamatergic synapse; / >>Dopaminergic synapse; / >>Long-term depression; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Spinocerebellar ataxia; / >>Pathways of neurodegeneration - multiple diseases; / >>Cocaine addiction; / >>Amphetamine addiction; / >>Nicotine addiction |
| Function | Function:Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.,miscellaneous:The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.,PTM:Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.,RNA editing:Partially edited. Fully edited in the brain. Heteromerically expressed edited GLUR2 (R) receptor complexes are impermeable to calcium, whereas the unedited (Q) forms are highly permeable to divalent ions.,similarity:Belongs to the glutamate-gated ion channel (TC 1.A.10) family.,subunit:Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4. Interacts with PRKCABP, GRIP1 and GRIP2., |
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