Name: GluR-3 Monoclonal Antibody
SKU: WM0219
Price: 268 USD
Availability: InStock

Product details

Background:
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	GluR-3
Reactivity	Human
Applications	WB, IHC, IF, ELISA
MW(Calculated)	101kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	19A12
Immunogen	Purified recombinant fragment of human GluR-3 expressed in E. Coli.
Specificity	GluR-3 Monoclonal Antibody detects endogenous levels of GluR-3 protein.
Gene Name	GRIA3
Protein Name	Glutamate receptor 3
Other Name	GRIA3; / GLUR3; / GLURC; / Glutamate receptor 3; / GluR-3; / AMPA-selective glutamate receptor 3; / GluR-C; / GluR-K3; / Glutamate receptor ionotropic; / AMPA 3; / GluA3
Species	Human
Gene ID	2892
Uniprot	P42263,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q9Z2W9
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Interaction with CNIH2 and CNIH3 promotes cell surface expression. .
Tissue Expression	Brain,Hippocampus,Skin,
Signaling_pathway	Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway
Research Areas	>>cAMP signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>Circadian entrainment; / >>Retrograde endocannabinoid signaling; / >>Glutamatergic synapse; / >>Dopaminergic synapse; / >>Long-term depression; / >>Huntington disease; / >>Spinocerebellar ataxia; / >>Pathways of neurodegeneration - multiple diseases; / >>Amphetamine addiction; / >>Nicotine addiction; / >>Transcriptional misregulation in cancer
Function	Caution:It is uncertain whether Met-1 or Met-7 is the initiator.,Disease:Defects in GRIA3 are the cause of mental retardation X-linked type 94 (MRX94) [MIM:300699]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRX94 patients have moderate mental retardation. Other variable features are macrocephaly, seizures, myoclonic jerks, autistic behavior, asthenic body habitus, distal muscle weakness and hyporeflexia.,Function:Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.,miscellaneous:The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.,PTM:Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-621 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-847 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.,similarity:Belongs to the glutamate-gated ion channel (TC 1.A.10) family.,subunit:Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with PRKCABP, GRIP1 and GRIP2.,