Price range: $268.00 through $328.00
Background:
This gene encodes an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in this gene are associated with rhizomelic chondrodysplasia punctata. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015],
| Size | 50μL/100μL |
| Target | GNPAT |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 77kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GNPAT. AA range:231-280 |
| Specificity | GNPAT Polyclonal Antibody detects endogenous levels of GNPAT protein. |
| Gene Name | GNPAT |
| Protein Name | Dihydroxyacetone phosphate acyltransferase |
| Other Name | GNPAT; / DAPAT; / DHAPAT; / Dihydroxyacetone phosphate acyltransferase; / DAP-AT; / DHAP-AT; / Acyl-CoA:dihydroxyacetonephosphateacyltransferase; / Glycerone-phosphate O-acyltransferase |
| Species | Human |
| Gene ID | 8443 |
| Uniprot | O15228, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P98192 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Peroxisome membrane; Peripheral membrane protein; Matrix side . Exclusively localized to the lumenal side of the peroxisomal membrane. . |
| Tissue Expression | Aorta endothelial cell,Brain,Liver,Lung,Thymus, |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Peroxisome |
| Research Areas | >>Glycerophospholipid metabolism; / >>Peroxisome |
| Function | Catalytic activity:Acyl-CoA + glycerone phosphate = CoA + acylglycerone phosphate.,Disease:Defects in GNPAT are the cause of rhizomelic chondrodysplasia punctata type 2 (RCDP2) [MIM:222765]. RDCP2 is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation.,Domain:The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.,pathway:Membrane lipid metabolism; glycerophospholipid metabolism.,similarity:Belongs to the GPAT/DAPAT family.,subcellular location:Exclusively localized to the lumenal side of the peroxisomal membrane.,subunit:May be part of an heterotrimeric complex composed of DAP-AT, ADAP-S and a modified form of DAP-AT., |
