Price range: $268.00 through $328.00
Background:
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013],
| Size | 50μL/100μL |
| Target | GOT2 |
| Reactivity | Human, Mouse |
| Applications | WB |
| MW(Calculated) | |
| MW(Observed) | 47kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 10C10 |
| Immunogen | Recombinant protein corresponding to fragment (aa/225-430) GOT2 . |
| Specificity | This antibody detects endogenous levels of GOT2. |
| Gene Name | GOT2 |
| Protein Name | |
| Other Name | GOT2; / Aspartate aminotransferase; / mitochondrial; / mAspAT; / Fatty acid-binding protein; / FABP-1; / Glutamate oxaloacetate transaminase 2; / Kynurenine aminotransferase 4; / Kynurenine aminotransferase IV; / Kynurenine--oxoglutarate transaminase 4; / Kynu |
| Species | Human |
| Gene ID | 2806 |
| Uniprot | P00505, |
| Species.1 | Mouse |
| Gene ID.1 | 14719 |
| Uniprot.1 | P05202 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Mitochondrion matrix . Cell membrane . Exposure to alcohol promotes translocation to the cell membrane. . |
| Tissue Expression | Epithelium,Gastric mucosa,Muscle, |
| Signaling_pathway | |
| Research Areas | >>Arginine biosynthesis; / >>Alanine, aspartate and glutamate metabolism; / >>Cysteine and methionine metabolism; / >>Arginine and proline metabolism; / >>Tyrosine metabolism; / >>Phenylalanine metabolism; / >>Phenylalanine, tyrosine and tryptophan biosynthesis; / >>Metabolic pathways; / >>Carbon metabolism; / >>2-Oxocarboxylic acid metabolism; / >>Biosynthesis of amino acids; / >>Fat digestion and absorption |
| Function | Catalytic activity:L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.,cofactor:Pyridoxal phosphate.,Function:Facilitates cellular uptake of long-chain free fatty acids.,miscellaneous:In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.,similarity:Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.,subunit:Homodimer., |
