SKU: WM0966 Categories: ,

His-Tag Monoclonal Antibody [4E6]

Price range: $268.00 through $328.00

SKU: WM0966-50
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Product details

Background:
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Specifications

Specification50μL;100μL
TargetHis-Tag
ReactivitySpecies independent
ApplicationsWB, IP, IF
MW(Calculated)
MW(Observed)
Host SpeciesMouse
Isotype
Conjugate
ModificationUnmodified
Recommended Dilution RatioWB 1:3000;IP 1:200;IF 1:1000
CompositionPBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Source
Purification ProcessThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Puity
Storage Condition-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone Number4E6
ImmunogenSynthetic peptide:HHHHHH conjugated to KLH.
Sequence
SpecificityThe antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.
Gene NameHis-Tag
Protein NameHis Tag
Alias
Organism-1
Gene ID-1
SwissProt-1
Organism-2
Gene ID-2
SwissProt-2
Organism-3
Gene ID-3
SwissProt-3
Organism-4
Gene ID-4
SwissProt-4
Cellular Localization
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