HPRT Monoclonal Antibody
Ucallm_WM0237-50
Price range: $268.00 through $328.00
Product details
Background:
hypoxanthine phosphoribosyltransferase 1(HPRT1) Homo sapiens The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009],
Specifications
| Size | 50μL/100μL |
| Target | HPRT |
| Reactivity | Human |
| Applications | WB, ELISA |
| MW(Calculated) | 25kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 20H5 |
| Immunogen | Purified recombinant fragment of HPRT expressed in E. Coli. |
| Specificity | HPRT Monoclonal Antibody detects endogenous levels of HPRT protein. |
| Gene Name | HPRT1 |
| Protein Name | Hypoxanthine-guanine phosphoribosyltransferase |
| Other Name | HPRT1; / HPRT; / Hypoxanthine-guanine phosphoribosyltransferase; / HGPRT; / HGPRTase |
| Species | Human |
| Gene ID | 3251 |
| Uniprot | P00492, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P00493 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm. |
| Tissue Expression | Brain, |
| Signaling_pathway | |
| Research Areas | >>Purine metabolism; / >>Drug metabolism - other enzymes; / >>Metabolic pathways; / >>Nucleotide metabolism |
| Function | Catalytic activity:GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.,Catalytic activity:IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.,cofactor:Binds 2 magnesium ions per subunit. One of the ions does not make direct protein contacts.,Disease:Defects in HPRT1 are the cause of gout [MIM:300323]; also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia.,Disease:Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS) [MIM:300322]. LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation.,online information:Hypoxanthine-guanine phosphoribosyltransferase entry,pathway:Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.,similarity:Belongs to the purine/pyrimidine phosphoribosyltransferase family.,subunit:Homotetramer., |
Please login to post questions
Related products
