Price range: $268.00 through $328.00
Background:
The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],
| Size | 50μL/100μL |
| Target | HRI |
| Reactivity | Human, Mouse, Monkey |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 71kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:100-300; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EIF2AK1. AA range:571-620 |
| Specificity | HRI Polyclonal Antibody detects endogenous levels of HRI protein. |
| Gene Name | EIF2AK1 |
| Protein Name | Eukaryotic translation initiation factor 2-alpha kinase 1 |
| Other Name | EIF2AK1; / HRI; / KIAA1369; / Eukaryotic translation initiation factor 2-alpha kinase 1; / Heme-controlled repressor; / HCR; / Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; / Heme-regulated inhibitor; / Hemin-sensitive initiation factor 2 |
| Species | Human |
| Gene ID | 27102 |
| Uniprot | Q9BQI3, |
| Species.1 | Mouse |
| Gene ID.1 | 15467 |
| Uniprot.1 | Q9Z2R9 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | cytoplasm, |
| Tissue Expression | Expressed predominantly in erythroid cells (PubMed:20071449). Expressed at much lower levels in hepatocytes (at protein level) (PubMed:20071449). |
| Signaling_pathway | |
| Research Areas | >>Protein processing in endoplasmic reticulum; / >>Hepatitis C; / >>Measles; / >>Herpes simplex virus 1 infection |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Hemin inactivates EIF2AK1 by promoting the formation of a disulfide-linked homodimer. Binding of nitric oxide (NO) to the heme iron in the N-terminal heme-binding domain activates the kinase activity, while binding of carbon monoxide (CO) suppresses kinase activity.,Function:Mediates down-regulation of protein synthesis in response to various stress conditions by the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51'. Protein synthesis is inhibited at the level of initiation.,miscellaneous:Can bind 2 molecules of heme per polypeptide chain.,PTM:Activated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 HRM (heme regulatory motif) repeats.,subunit:Synthesized in an inactive form that binds to the N-terminal domain of CDC37. Has to be associated with a multiprotein complex containing Hsp90, CDC37 and PPP5C for maturation and activation by autophosphorylation. The phosphatase PPP5C modulates this activation. Homodimer; non-covalently bound in the absence of hemin. Converted to an inactive disulfide linked homodimer in the presence of hemin.,tissue specificity:Detected in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, muscle and stomach., |
