Hrs Polyclonal Antibody
Ucallm_WP12746-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010],
Specifications
| Size | 50μL/100μL |
| Target | Hrs |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 80kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HRS. AA range:301-350 |
| Specificity | Hrs Polyclonal Antibody detects endogenous levels of Hrs protein. |
| Gene Name | HGS |
| Protein Name | Hepatocyte growth factor-regulated tyrosine kinase substrate |
| Other Name | HGS; / HRS; / Hepatocyte growth factor-regulated tyrosine kinase substrate; / Hrs; / Protein pp110 |
| Species | Human |
| Gene ID | 9146 |
| Uniprot | O14964, |
| Species.1 | Mouse |
| Gene ID.1 | 15239 |
| Uniprot.1 | Q99LI8, |
| Species.2 | Rat |
| Gene ID.2 | 56084 |
| Uniprot.2 | Q9JJ50 |
| Cellular Localization | Cytoplasm . Early endosome membrane; Peripheral membrane protein; Cytoplasmic side . Endosome, multivesicular body membrane; Peripheral membrane protein . Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments. . |
| Tissue Expression | Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Phagosome |
| Research Areas | >>Viral life cycle - HIV-1; / >>Endocytosis; / >>Phagosome |
| Function | Domain:Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.,Function:Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex.,PTM:Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.,similarity:Contains 1 FYVE-type zinc finger.,similarity:Contains 1 UIM (ubiquitin-interacting motif) repeat.,similarity:Contains 1 VHS domain.,subunit:Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 (or probably STAM) and EPS15. Interacts with STAM. Interacts with STAM2. Interacts with EPS15; the interaction is direct, calcium-dependent and inhibited by SNAP25. Interacts with NF2; the interaction is direct. Interacts with ubiquitin; the interaction is direct. Interacts with VPS37C. Interacts with SMAD1, SMAD2 and SMAD3. Interacts with TSG101; the interaction mediates the association with the ESCRT-I complex. Interacts with SNAP25; the interaction is direct and decreases with addition of increasing concentrations of free calcium. Interacts with SNX1; the interaction is direct. Component of a 550 kDa membrane complex at least composed of HGS and SNX1 but excluding EGFR.,tissue specificity:Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes., |
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