Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008],
| Size | 50μL/100μL |
| Target | HSP27 |
| Reactivity | Human, Monkey |
| Applications | WB, ICC |
| MW(Calculated) | |
| MW(Observed) | 27kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000; ICC 1:300 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 7H8 |
| Immunogen | Purified recombinant human Hsp27 protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of Hsp27 and does not cross-react with related proteins. |
| Gene Name | HSPB1 HSP27 HSP28 |
| Protein Name | |
| Other Name | Heat shock 27kDa protein; / 28 kDa heat shock protein; / CMT2F; / DKFZp586P1322; / Estrogen regulated 24 kDa protein; / Estrogen-regulated 24 kDa protein; / Heat shock 25kDa protein 1; / Heat shock 27 kDa protein; / Heat shock 27kD protein 1; / Heat shock 27kDa protein 1; / Heat shock 28kDa protein 1; / Heat Shock Protein 27; / Heat Shock Protein 27; / Heat shock protein beta 1; / Heat shock protein beta-1; / HMN2B; / HS.76067; / Hsp 25; / HSP 27; / Hsp 28; / Hsp B1; / Hsp25; / HSP27; / Hsp28; / HspB1; / HSPB1_HUMAN; / SRP27; / Stress responsive protein 27; / Stress-responsive protein 27. |
| Species | Human |
| Gene ID | 3315 |
| Uniprot | P04792, |
| Species.1 | Mouse |
| Gene ID.1 | 15507 |
| Uniprot.1 | P14602 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. . |
| Tissue Expression | Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway |
| Research Areas | >>MAPK signaling pathway; / >>VEGF signaling pathway; / >>Amoebiasis |
| Function | Disease:Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.,Disease:Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.,Function:Involved in stress resistance and actin organization.,induction:Expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells.,PTM:Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.,similarity:Belongs to the small heat shock protein (HSP20) family.,subcellular location:Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.,subunit:Interacts with TGFB1I1 (By similarity). Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8 and HSPBAP1.,tissue specificity:Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle., |
