SKU: WP12762 Categories: ,

HSP40 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP12762-100
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Product details

Background:

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],

Specifications

Size50μL/100μL
TargetHSP40
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)45kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human HSP40. AA range:271-320
SpecificityHSP40 Polyclonal Antibody detects endogenous levels of HSP40 protein.
Gene NameDNAJB1
Protein NameDnaJ homolog subfamily B member 1
Other NameDNAJB1; / DNAJ1; / HDJ1; / HSPF1; / DnaJ homolog subfamily B member 1; / DnaJ protein homolog 1; / Heat shock 40 kDa protein 1; / HSP40; / Heat shock protein 40; / Human DnaJ protein 1; / hDj-1
SpeciesHuman
Gene ID3337
UniprotP25685,
Species.1Mouse
Gene ID.181489
Uniprot.1Q9QYJ3
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCytoplasm . Nucleus . Nucleus, nucleolus . Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
Tissue ExpressionBrain,Heart,Lung,Placenta,
Signaling_pathwayGenetic Information Processing >> Folding, sorting and degradation >> Protein processing in endoplasmic reticulum
Research Areas>>Protein processing in endoplasmic reticulum; / >>Influenza A
FunctionFunction:Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.,induction:By heat shock.,similarity:Contains 1 J domain.,subcellular location:Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.,subunit:Interacts with DNAJC3.,
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