U-Blot® HXK I Rabbit mAb
Ucallm_WR4854-50
Price range: $268.00 through $328.00
Product details
Background:Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016],
Specifications
| Size | 50μL/100μL |
| Target | HXK I |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 103kD |
| MW(Observed) | 103kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1018R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | HK1 |
| Protein Name | Hexokinase-1 |
| Other Name | HK1; / Hexokinase-1; / Brain form hexokinase; / Hexokinase type I; / HK I |
| Species | Human |
| Gene ID | 3098 |
| Uniprot | P19367, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P17710, |
| Species.2 | Rat |
| Gene ID.2 | 25058 |
| Uniprot.2 | P05708 |
| Cellular Localization | Mitochondrion outer membrane; Peripheral membrane protein . Cytoplasm, cytosol . The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable). Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331). . |
| Tissue Expression | Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific (PubMed:10978502). Isoform 4: Testis-specific (PubMed:10978502). |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin signaling pathway;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway |
| Research Areas | >>Glycolysis / Gluconeogenesis; / >>Fructose and mannose metabolism; / >>Galactose metabolism; / >>Starch and sucrose metabolism; / >>Amino sugar and nucleotide sugar metabolism; / >>Neomycin, kanamycin and gentamicin biosynthesis; / >>Metabolic pathways; / >>Carbon metabolism; / >>Biosynthesis of nucleotide sugars; / >>HIF-1 signaling pathway; / >>Insulin signaling pathway; / >>Type II diabetes mellitus; / >>Carbohydrate digestion and absorption; / >>Shigellosis; / >>Central carbon metabolism in cancer |
| Function | Catalytic activity:ATP + D-hexose = ADP + D-hexose 6-phosphate.,Disease:Defects in HK1 are the cause of hexokinase deficiency [MIM:235700]. Hexokinase deficiency is a rare autosomal recessive disease with nonspherocytic hemolytic anemia as the predominant clinical feature.,Domain:The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.,enzyme regulation:Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.,miscellaneous:In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).,online information:Hexokinase entry,pathway:Carbohydrate metabolism; hexose metabolism.,similarity:Belongs to the hexokinase family.,subcellular location:Its hydrophobic N-terminal sequence may be involved in membrane binding.,subunit:Monomer.,tissue specificity:Isoform 2 is erythrocyte specific; isoform 3 and isoform 4 are testis-specific., |
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