Price range: $268.00 through $328.00
Background:
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009],
| Size | 50μL/100μL |
| Target | HXK III |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 99kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Hexokinase-3. AA range:811-860 |
| Specificity | HXK III Polyclonal Antibody detects endogenous levels of HXK III protein. |
| Gene Name | HK3 |
| Protein Name | Hexokinase-3 |
| Other Name | HK3; / Hexokinase-3; / Hexokinase type III; / HK III |
| Species | Human |
| Gene ID | 3101 |
| Uniprot | P52790, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q3TRM8 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | cell,mitochondrion,cytosol, |
| Tissue Expression | Blood,Liver,Spleen, |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin signaling pathway;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway |
| Research Areas | >>Glycolysis / Gluconeogenesis; / >>Fructose and mannose metabolism; / >>Galactose metabolism; / >>Starch and sucrose metabolism; / >>Amino sugar and nucleotide sugar metabolism; / >>Neomycin, kanamycin and gentamicin biosynthesis; / >>Metabolic pathways; / >>Carbon metabolism; / >>Biosynthesis of nucleotide sugars; / >>HIF-1 signaling pathway; / >>Insulin signaling pathway; / >>Type II diabetes mellitus; / >>Carbohydrate digestion and absorption; / >>Shigellosis; / >>Central carbon metabolism in cancer |
| Function | Catalytic activity:ATP + D-hexose = ADP + D-hexose 6-phosphate.,Domain:The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.,enzyme regulation:Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.,miscellaneous:In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).,online information:Hexokinase entry,pathway:Carbohydrate metabolism; hexose metabolism.,similarity:Belongs to the hexokinase family.,subunit:Monomer., |
