I12R2 Polyclonal Antibody
Ucallm_WP2170-50
Price range: $268.00 through $328.00
Product details
Background:
interleukin 12 receptor subunit beta 2(IL12RB2) Homo sapiens The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. Several transcript variants encoding different isoforms and non-protein coding transcripts have been found for this gene. [provided by RefSeq, Apr 2012],
Specifications
| Size | 50μL/100μL |
| Target | I12R2 |
| Reactivity | Human, Mouse |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 94kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 270-350 |
| Specificity | I12R2 Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | IL12RB2 |
| Protein Name | Interleukin-12 receptor subunit beta-2 (IL-12 receptor subunit beta-2) (IL-12R subunit beta-2) (IL-12R-beta-2) (IL-12RB2) |
| Other Name | |
| Species | Human |
| Gene ID | 3595 |
| Uniprot | Q99665, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P97378 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
| Tissue Expression | Isoform 2 is expressed at similar levels in both naive and activated T-cells. |
| Signaling_pathway | Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Immune disease >> Inflammatory bowel disease;Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cytokine-cytokine receptor interaction |
| Research Areas | >>Cytokine-cytokine receptor interaction; / >>JAK-STAT signaling pathway; / >>Th1 and Th2 cell differentiation; / >>Pathways in cancer; / >>Inflammatory bowel disease |
| Function | developmental stage:Maximum levels in Th1 cells between day 3 and day 8 of activation.,Domain:The box 1 motif is required for JAK interaction and/or activation.,Domain:The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.,Function:Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.,induction:In vitro, up-regulated by interferon alpha.,polymorphism:Heterozygotic variants Gly-313 and Arg-720 are associated with atopy, an immunological condition that can lead to clinical symptoms such as allergic rhinitis, sinusitis, asthma and eczema.,PTM:On IL12 binding, phosphorylated on C-terminal tyrosine residues by JAK2. Phosphorylation on Tyr-800 is required for STAT4 binding and activation, and for SOCS3 binding.,similarity:Belongs to the type I cytokine receptor family. Type 2 subfamily.,similarity:Contains 5 fibronectin type-III domains.,subunit:Heterodimer/heterooligomer; disulfide-linked. The functional high affinity IL12 receptor is composed of I12RB1 and IL12RB2. Il12RB2 binds JAK2 (via its N-terminal) through a membrane-proximal region of the cytoplasmic domain. Interaction, in vitro and in vivo, with SOCS3 (via its SH2 domain) inhibits the STAT4-mediated activation. Binds STAT4 through a membrane-distal C-terminal region.,tissue specificity:Isoform 2 is expressed at similar levels in both naive and activated T-cells., |
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