SKU: WP12772 Categories: ,

IARS2 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP12772-100
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Product details

Background:

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014],

Specifications

Size50μL/100μL
TargetIARS2
ReactivityHuman, Mouse, Monkey
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)114kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human IARS2. AA range:71-120
SpecificityIARS2 Polyclonal Antibody detects endogenous levels of IARS2 protein.
Gene NameIARS2
Protein NameIsoleucine--tRNA ligase mitochondrial
Other NameIARS2; / Isoleucine--tRNA ligase; / mitochondrial; / Isoleucyl-tRNA synthetase; / IleRS
SpeciesHuman
Gene ID55699
UniprotQ9NSE4,
Species.1Mouse
Gene ID.1381314
Uniprot.1Q8BIJ6
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationMitochondrion matrix .
Tissue ExpressionBrain,Epithelium,Liver,Placenta,Skin,
Signaling_pathway
Research Areas>>Aminoacyl-tRNA biosynthesis
FunctionCatalytic activity:ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).,similarity:Belongs to the class-I aminoacyl-tRNA synthetase family.,
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