SKU: WP3320 Categories: ,

IF5A1 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP3320-100
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Product details

Background:

function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond., PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine)., similarity:Belongs to the eIF-5A family., subunit:Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4., tissue specificity:Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).,

Specifications

Size50μL/100μL
TargetIF5A1
ReactivityHuman, Mouse, Rat
ApplicationsWB, ELISA
MW(Calculated)
MW(Observed)16kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-2000; ELISA 1:5000-20000
FormulationLiquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenSynthesized peptide derived from part region of human protein
SpecificityIF5A1 Polyclonal Antibody detects endogenous levels of protein.
Gene NameEIF5A
Protein NameEukaryotic translation initiation factor 5A-1 (eIF-5A-1) (eIF-5A1) (Eukaryotic initiation factor 5A isoform 1) (eIF-5A) (Rev-binding factor) (eIF-4D)
Other Name
SpeciesHuman
Gene ID1984
UniprotP63241,
Species.1Mouse
Gene ID.1
Uniprot.1P63242,
Species.2Rat
Gene ID.2
Uniprot.2Q3T1J1
Cellular LocalizationCytoplasm . Nucleus . Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side . Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions (PubMed:19379712, PubMed:27306458). Nuclear export of hypusinated protein is mediated by XPO4 (PubMed:10944119, PubMed:27306458). .
Tissue ExpressionExpressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
Signaling_pathway
Research Areas
FunctionFunction:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,subunit:Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4.,tissue specificity:Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).,
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