Name: Ikaros [C-term] mouse mAb
SKU: WM0606
Price: 268 USD
Availability: InStock

Product details

Background:
This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding

Specifications

Target	Ikaros
Reactivity	Human
Applications	WB, IP
MW(Calculated)
MW(Observed)	58kD
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:1000
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	1A12
Immunogen	Purified recombinant human Ikaros(C-terminus) protein fragments expressed in E.coli.
Sequence
Specificity	This antibody detects endogenous levels of Ikaros(C-terminus) and does not cross-react with related proteins.
Gene Name	IKZF1
Protein Name
Other Name	CLL associated antigen KW 6; / DNA-binding protein Ikaros; / hIk 1; / hIk-1; / Hs.54452; / IK1; / Ikaros; / zinc finger protein; / IKAROS; / IKAROS family zinc finger 1; / Ikaros; / Ikaros family zinc finger protein 1; / Ikzf1; / IKZF1_HUMAN; / LYF1; / Lymphoid transcription factor LyF-1; / PRO0758; / Zinc finger protein subfamily 1A 1; / Ikaros; / zinc finger protein subfamily 1A 1; / Zinc finger protein, subfamily 1A, member 1; / ZNFN1A1.
Species	Human
Gene ID-1	10320
Uniprot	Q13422,
Species.1	Mouse
Gene ID-2
Uniprot.1	Q03267
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014]
Cellular Localization	Nucleus . In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events. .; [Isoform Ik2]: Nucleus. In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events (By similarity). .; [Isoform Ik6]: Cytoplasm .
Tissue Expression	Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.
Signaling_pathway
Research Areas
Function	Function:Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Functions in the specification and the maturation of the T-lymphocyte. Also interacts with a critical control element in the TDT (terminal deoxynucleotidyltransferase) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development.,similarity:Belongs to the Ikaros C2H2-type zinc-finger protein family.,similarity:Contains 6 C2H2-type zinc fingers.,subunit:Interacts with IKZF4 AND IKZF5.,tissue specificity:Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.,
RRID