INSIG1 rabbit pAb
Ucallm_WP6318-50
Price range: $268.00 through $328.00
Product details
Background:
Specifications
| Size | 50μL/100μL |
| Target | INSIG1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| MW(Calculated) | 30kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human INSIG1 |
| Specificity | This antibody detects endogenous levels of INSIG1 at Human, Mouse,Rat |
| Gene Name | INSIG1 |
| Protein Name | Insulin-induced gene 1 protein (INSIG-1) |
| Other Name | |
| Species | Human |
| Gene ID | 3638 |
| Uniprot | O15503, |
| Species.1 | Mouse |
| Gene ID.1 | 231070 |
| Uniprot.1 | Q8BGI3, |
| Species.2 | Rat |
| Gene ID.2 | 64194 |
| Uniprot.2 | Q08755 |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein . |
| Tissue Expression | Expressed in all tissues tested with highest expression in the liver. |
| Signaling_pathway | |
| Research Areas | |
| Function | Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR . Acts as a negative regulator of cholesterol biosynthesis by mediating the retention of the SCAP-SREBP complex in the endoplasmic reticulum, thereby blocking the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 . Binds oxysterol, including 25-hydroxycholesterol, regulating interaction with SCAP and retention of the SCAP-SREBP complex in the endoplasmic reticulum . In presence of oxysterol, interacts with SCAP, retaining the SCAP-SREBP complex in the endoplasmic reticulum, thereby preventing SCAP from escorting SREBF1/SREBP1 and SREBF2/SREBP2 to the Golgi . Sterol deprivation or phosphorylation by PCK1 reduce oxysterol-binding, disrupting the interaction between INSIG1 and SCAP, thereby promoting Golgi transport of the SCAP-SREBP complex, followed by processing and nuclear translocation of SREBF1/SREBP1 and SREBF2/SREBP2 . Also regulates cholesterol synthesis by regulating degradation of HMGCR: initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligases AMFR/gp78 and/or RNF139 . Also regulates degradation of SOAT2/ACAT2 when the lipid levels are low: initiates the ubiquitin-mediated degradation of SOAT2/ACAT2 via recruitment of the ubiquitin ligases AMFR/gp78 . |
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