Insulin [ABT-INS] mouse mAb
Ucallm_WM1580-50
Price range: $268.00 through $328.00
Product details
Background:
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010],
Specifications
| Size | 50μL/100μL |
| Target | Insulin |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, ELISA |
| MW(Calculated) | 12kD |
| MW(Observed) | 9kD |
| Host Species | Mouse |
| Isotype | IgG2b,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-400; ELISA 1:500-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM-INS |
| Immunogen | Synthesized peptide derived from human Insulin AA range: 25-110 |
| Specificity | The antibody can specifically recognize human Insulin protein. |
| Gene Name | INS |
| Protein Name | Insulin [Cleaved into: Insulin B chain; Insulin A chain] |
| Other Name | |
| Species | Human |
| Gene ID | 3630 |
| Uniprot | P01308 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic |
| Tissue Expression | Blood,Liver,Muscle,Pancreas, |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Endocrine system >> Insulin secretion;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Prolactin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway |
| Research Areas | >>MAPK signaling pathway; / >>Ras signaling pathway; / >>Rap1 signaling pathway; / >>cGMP-PKG signaling pathway; / >>HIF-1 signaling pathway; / >>FoxO signaling pathway; / >>Phospholipase D signaling pathway; / >>Oocyte meiosis; / >>Autophagy - animal; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Regulation of actin cytoskeleton; / >>Insulin signaling pathway; / >>Insulin secretion; / >>Ovarian steroidogenesis; / >>Progesterone-mediated oocyte maturation; / >>Prolactin signaling pathway; / >>Regulation of lipolysis in adipocytes; / >>Type II diabetes mellitus; / >>Insulin resistance; / >>Non-alcoholic fatty liver disease; / >>Type I diabetes mellitus; / >>Maturity onset diabetes of the young; / >>Aldosterone-regulated sodium reabsorption; / >>Alzheimer disease; / >>Prostate cancer; / >>Diabetic cardiomyopathy |
| Function | Disease:Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].,Function:Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.,Function:Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.,Function:The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.,mass spectrometry: PubMed:12586351; PubMed:15359740,online information:Clinical information on Eli Lilly insulin products,online information:Insulin entry,online information:Insulin-like growth factor 2 entry,online information:Protein of the 20th century - Issue 9 of April 2001,pharmaceutical:Available under the names Humulin or Humalog (Eli Lilly) and Novolin (Novo Nordisk). Used in the treatment of diabetes. Humalog is an insulin analog with 52-Lys-Pro-53 instead of 52-Pro-Lys-53.,similarity:Belongs to the insulin family.,subunit:Heterodimer of a B chain and an A chain linked by two disulfide bonds., |
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