Name: Insulin Polyclonal Antibody
SKU: WP12840
Price: 268 USD
Availability: InStock

Product details

Background:

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010],

Specifications

Target	Insulin
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	12kD
MW(Observed)
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human Insulin. AA range:49-98
Sequence
Specificity	Insulin Polyclonal Antibody detects endogenous levels of Insulin protein.
Gene Name	INS
Protein Name	Insulin
Other Name	INS; / Insulin
Species	Human
Gene ID-1	3630
Uniprot	P01308
Species.1
Gene ID-2
Uniprot.1
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010],
Cellular Localization	Secreted.
Tissue Expression	Blood,Liver,Muscle,Pancreas,
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Endocrine system >> Insulin secretion;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Prolactin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas	>>MAPK signaling pathway; / >>Ras signaling pathway; / >>Rap1 signaling pathway; / >>cGMP-PKG signaling pathway; / >>HIF-1 signaling pathway; / >>FoxO signaling pathway; / >>Phospholipase D signaling pathway; / >>Oocyte meiosis; / >>Autophagy - animal; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Regulation of actin cytoskeleton; / >>Insulin signaling pathway; / >>Insulin secretion; / >>Ovarian steroidogenesis; / >>Progesterone-mediated oocyte maturation; / >>Prolactin signaling pathway; / >>Regulation of lipolysis in adipocytes; / >>Type II diabetes mellitus; / >>Insulin resistance; / >>Non-alcoholic fatty liver disease; / >>Type I diabetes mellitus; / >>Maturity onset diabetes of the young; / >>Aldosterone-regulated sodium reabsorption; / >>Alzheimer disease; / >>Prostate cancer; / >>Diabetic cardiomyopathy
Function	Disease:Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].,Function:Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.,Function:Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.,Function:The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.,mass spectrometry: PubMed:12586351; PubMed:15359740,online information:Clinical information on Eli Lilly insulin products,online information:Insulin entry,online information:Insulin-like growth factor 2 entry,online information:Protein of the 20th century - Issue 9 of April 2001,pharmaceutical:Available under the names Humulin or Humalog (Eli Lilly) and Novolin (Novo Nordisk). Used in the treatment of diabetes. Humalog is an insulin analog with 52-Lys-Pro-53 instead of 52-Pro-Lys-53.,similarity:Belongs to the insulin family.,subunit:Heterodimer of a B chain and an A chain linked by two disulfide bonds.,
RRID

Please login to post questions

Insulin Polyclonal Antibody

Product details

Specifications

About Us

Support

Order

Menu

Insulin Polyclonal Antibody

Product details

Specifications

Related products

U-Blot® α smooth muscle actin Rabbit mAb

U-Blot® NQO1 Rabbit mAb

Acetyl Lysine Monoclonal Antibody [10B10]

U-Blot® CDK6 Rabbit mAb

U-Blot™ SSPE buffer, pH 7.4

U-Blot® Ezrin Rabbit mAb

Customer Reviews

Menu