Name: Integrin β3 [PTR2555] mouse mAb
SKU: WM1337
Price: 268 USD
Availability: InStock

Product details

Background:
integrin subunit beta 3(ITGB3) Homo sapiens The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008],

Specifications

Target	Integrin β3
Reactivity	Human, Mouse, Rat
Applications	WB, IF, ELISA
MW(Calculated)	87kD
MW(Observed)	72kD
Host Species	Mouse
Isotype	IgG1,Lambda
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	PTR2555
Immunogen	Synthesized peptide derived from human Integrin β3 AA range: 700-788
Sequence
Specificity	This antibody detects endogenous levels of Integrin β3 protein.
Gene Name	ITGB3 GP3A
Protein Name	Integrin beta-3 (Platelet membrane glycoprotein IIIa) (GPIIIa) (CD antigen CD61)
Other Name	Integrin beta-3; / Platelet membrane glycoprotein IIIa; / GPIIIa; / CD antigen CD61
Species	Human
Gene ID-1	3690
Uniprot	P05106,
Species.1	Mouse
Gene ID-2	16416
Uniprot.1	O54890
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	integrin subunit beta 3(ITGB3) Homo sapiens The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008],
Cellular Localization
Tissue Expression	Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Phagosome;Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> ECM-receptor interaction
Research Areas	>>Rap1 signaling pathway; / >>Phagosome; / >>PI3K-Akt signaling pathway; / >>Osteoclast differentiation; / >>Focal adhesion; / >>ECM-receptor interaction; / >>Platelet activation; / >>Neutrophil extracellular trap formation; / >>Hematopoietic cell lineage; / >>Regulation of actin cytoskeleton; / >>Thyroid hormone signaling pathway; / >>Human cytomegalovirus infection; / >>Human papillomavirus infection; / >>Herpes simplex virus 1 infection; / >>Proteoglycans in cancer; / >>MicroRNAs in cancer; / >>Hypertrophic cardiomyopathy; / >>Arrhythmogenic right ventricular cardiomyopathy; / >>Dilated cardiomyopathy; / >>Fluid shear stress and atherosclerosis
Function	Disease:Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. Its inheritance is autosomal recessive. It is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb-IIIa complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the GPIIb-IIIa complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT variants have normal or near normal (60-100%) expression of dysfunctional receptors.,Function:Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.,online information:The Singapore human mutation and polymorphism database,polymorphism:Position 169 is associated with platelet-specific alloantigen HPA-4 (PEN or YUK). HPA-4A/PEN(A)/YUK(A) has Arg-169 and HPA-4B/PEN(B)/YUK(B) has Gln-169. HPA-4B is involved in neonatal alloimmune thrombocytopenia (NAIT or NATP).,polymorphism:Position 433 is associated with platelet-specific alloantigen MO. MO(-) has Pro-433 and MO(+) has Ala-433. MO(+) is involved in NAIT.,polymorphism:Position 515 is associated with platelet-specific alloantigen CA/TU. CA(-)/TU(-) has Arg-515 and CA(+)/TU(+) has Gln-515. CA(+) is involved in NAIT.,polymorphism:Position 59 is associated with platelet-specific alloantigen HPA-1 (ZW or PL(A)). HPA-1A/ZW(A)/PL(A1) has Leu-59 and HPA-1B/ZW(B)/PL(A2) has Pro-59.,polymorphism:Position 662 is associated with platelet-specific alloantigen SR(A). SR(A)(-) has Arg-662 and SR(A)(+) has Cys-662.,PTM:Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.,similarity:Belongs to the integrin beta chain family.,similarity:Contains 1 VWFA domain.,subunit:Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIb or alpha-V. Isoform Beta-3C interacts with FLNB. Interacts with HIV-1 Tat.,tissue specificity:Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.,
RRID