U-Blot® IRF-1 Rabbit mAb
WR4551 Mouse brain whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | IRF 1 |
| Reactivity | Human, Mouse, Rat, Fish |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 37kD |
| MW(Observed) | 48kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:400; WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0828R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | IRF1 |
| Protein Name | Interferon regulatory factor 1 |
| Other Name | IRF1; / Interferon regulatory factor 1; / IRF-1 |
| Species | Human |
| Gene ID | 3659 |
| Uniprot | P10914, |
| Species.1 | Mouse |
| Gene ID.1 | 16362 |
| Uniprot.1 | P15314, |
| Species.2 | Rat |
| Gene ID.2 | 24508 |
| Uniprot.2 | P23570 |
| Cellular Localization | Nucleus . Cytoplasm . MYD88-associated IRF1 migrates into the nucleus more efficiently than non-MYD88-associated IRF1. . |
| Tissue Expression | Pancreas,Placenta,Spleen, |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Prolactin signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway |
| Research Areas | >>C-type lectin receptor signaling pathway; / >>TNF signaling pathway; / >>Prolactin signaling pathway; / >>Pertussis; / >>Human papillomavirus infection |
| Function | Disease:Deletion or rearrangement of IRF1 are a cause of preleukemic myelodysplastic syndrome (MDS) and of acute myelogenous leukemia (AML).,Function:Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and activates those genes. Acts as a tumor suppressor.,induction:By viruses and IFN.,PTM:Sumoylation represses the transcriptional activity and displays enhanced resistance to protein degradation. Inactivates the tumor suppressor activity. Elevated levels in tumor cells. Major site is Lys-275. Sumoylation is enhanced by PIAS3 (By similarity). Desumoylated by SENP1 in tumor cells and appears to compete with ubiquitination on C-terminal sites.,PTM:Ubiquitinated. Appears to compete with sumoylation on C-terminal sites.,similarity:Belongs to the IRF family.,similarity:Contains 1 tryptophan pentad repeat DNA-binding domain., |
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