Price range: $268.00 through $328.00
Background:
This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | ITK |
| Reactivity | Human, Mouse |
| Applications | IHC, IF, WB |
| MW(Calculated) | |
| MW(Observed) | 72kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:50-200; WB 1:500-2000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human ITK AA range: 450-530 |
| Specificity | This antibody detects endogenous levels of human ITK |
| Gene Name | ITK EMT LYK |
| Protein Name | Tyrosine-protein kinase ITK/TSK |
| Other Name | ITK; / EMT; / LYK; / Tyrosine-protein kinase ITK/TSK; / Interleukin-2-inducible T-cell kinase; / IL-2-inducible T-cell kinase; / Kinase EMT; / T-cell-specific kinase; / Tyrosine-protein kinase Lyk |
| Species | Human |
| Gene ID | 3702 |
| Uniprot | Q08881, |
| Species.1 | Mouse |
| Gene ID.1 | 16428 |
| Uniprot.1 | Q03526 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Nucleus . Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation. |
| Tissue Expression | T-cell lines and natural killer cell lines. |
| Signaling_pathway | Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Immune system >> Chemokine signaling pathway |
| Research Areas | >>Chemokine signaling pathway; / >>T cell receptor signaling pathway; / >>Leukocyte transendothelial migration |
| Function | Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 1 zinc ion per subunit.,Function:Plays a role in T-cell proliferation and differentiation.,induction:By interleukin-2.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.,similarity:Contains 1 Btk-type zinc finger.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.,tissue specificity:T-cell lines and natural killer cell lines., |
