U-Blot® Lactate Dehydrogenase Rabbit mAb
WR4096 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4095 Western blot analysis of lysates from A431 cells, using Cathepsin D Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
WR4096 Immunohistochemical analysis of paraffin-embedded Rat liver tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4096 Immunohistochemical analysis of paraffin-embedded Human liver tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Price range: $268.00 through $328.00
Product details
Background:The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008],
Specifications
| Size | 50μL/100μL |
| Target | LDHA;LDHB;LDHC |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 37kD |
| MW(Observed) | 37kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:1000-3000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0186R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | LDHA; PIG19; LDHB; LDHC; LDH3; LDHX |
| Protein Name | L-lactate dehydrogenase A chain; LDH-A; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59; L-lactate dehydrogenase B chain; LDH-B; LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46; L-lactate dehydrogenase C chain; LDH-C; Cancer/testis antigen 32; CT32; LDH testis subunit; LDH-X |
| Other Name | LDHA; / PIG19; / LDHB; / LDHC; / LDH3; / LDHX; / L-lactate dehydrogenase A chain; / LDH-A; / Cell proliferation-inducing gene 19 protein; / LDH muscle subunit; / LDH-M; / Renal carcinoma antigen NY-REN-59; / L-lactate dehydrogenase B chain; / LDH-B; / LDH heart subunit; / LDH-H; / Renal carcinoma antigen NY-REN-46; / L-lactate dehydrogenase C chain; / LDH-C; / Cancer/testis antigen 32; / CT32; / LDH testis subunit; / LDH-X |
| Species | Human |
| Gene ID | 3939 |
| Uniprot | P00338;P07195;P07864, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P06151, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P04642 |
| Cellular Localization | Cytoplasmic, Membranous |
| Tissue Expression | Predominantly expressed in anaerobic tissues such as skeletal muscle and liver. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway |
| Research Areas | >>Glycolysis / Gluconeogenesis; / >>Cysteine and methionine metabolism; / >>Pyruvate metabolism; / >>Propanoate metabolism; / >>Metabolic pathways; / >>HIF-1 signaling pathway; / >>Glucagon signaling pathway; / >>Central carbon metabolism in cancer |
| Function | Catalytic activity:(S)-lactate + NAD(+) = pyruvate + NADH.,Caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,Disease:Defects in LDHA are a cause of exertional myoglobinuria.,online information:Lactate dehydrogenase entry,pathway:Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.,similarity:Belongs to the LDH/MDH superfamily.,similarity:Belongs to the LDH/MDH superfamily. LDH family.,subunit:Homotetramer., |
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