U-Blot® LKB1 Rabbit mAb
WR4362 HEK293 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
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Product details
Background:This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | LKB1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF, IP, ELISA |
| MW(Calculated) | 49kD |
| MW(Observed) | 60kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0574R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | STK11 |
| Protein Name | Serine/threonine-protein kinase STK11 |
| Other Name | STK11; / LKB1; / PJS; / Serine/threonine-protein kinase STK11; / Liver kinase B1; / LKB1; / hLKB1; / Renal carcinoma antigen NY-REN-19 |
| Species | Human |
| Gene ID | 6794 |
| Uniprot | Q15831, |
| Species.1 | Mouse |
| Gene ID.1 | 20869 |
| Uniprot.1 | Q9WTK7 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, Nucleus |
| Tissue Expression | Ubiquitously expressed. Strongest expression in testis and fetal liver. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway |
| Research Areas | >>FoxO signaling pathway; / >>Autophagy - animal; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Tight junction; / >>Adipocytokine signaling pathway |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium or manganese.,Disease:Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.,Disease:Defects in STK11 have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT).,enzyme regulation:Activated by binding of a complex consisting of CAB39 and STRAD or CAB39 and ALS2CR2.,Function:Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.,online information:PJS entry,PTM:Phosphorylated by a cAMP-dependent protein kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.,subunit:Found in a ternary complex composed of SMAD4, STK11 and STK11IP. Interacts with SMAD4 and STK11IP.,tissue specificity:Ubiquitously expressed. Strongest expression in testis and fetal liver., |
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