Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a phospholipase that hydrolyzes phosphatidic acid to produce lysophosphatidic acid. Defects in this gene are a cause of susceptibility to familial hypertrigliceridemia. This gene is also expressed at high levels in Ewing family tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014],
| Size | 50μL/100μL |
| Target | LPD lipase |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 53kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human LIPI. AA range:289-338 |
| Specificity | LPD lipase Polyclonal Antibody detects endogenous levels of LPD lipase protein. |
| Gene Name | LIPI |
| Protein Name | Lipase member I |
| Other Name | LIPI; / LPDL; / PRED5; / Lipase member I; / LIPI; / Cancer/testis antigen 17; / CT17; / LPD lipase; / Membrane-associated phosphatidic acid-selective phospholipase A1-beta; / mPA-PLA1 beta |
| Species | Human |
| Gene ID | 149998 |
| Uniprot | Q6XZB0 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | [Isoform 1]: Cell membrane . Secreted . May associate with lipid draft. .; [Isoform 2]: Cell membrane . Secreted . May associate with lipid draft. . |
| Tissue Expression | Expressed in testis. Expressed exclusively at the connecting piece of the sperm. |
| Signaling_pathway | |
| Research Areas | |
| Function | Disease:Defects in LIPI may be a cause of susceptibility to familial hypertrigliceridemia [MIM:145750]. Familial hypertriglyceridemia is a common inherited disorder in which the concentration of very low density lipoprotein (VLDL) is elevated in the plasma. This leads to increased risk of heart disease, obesity, and pancreatitis.,enzyme regulation:Inhibited by sodium vanadate.,Function:Hydrolyzes specifically phosphatidic acid (PA) to produce lysophosphatidic acid (LPA).,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the AB hydrolase superfamily. Lipase family.,subcellular location:May associate with lipid draft.,subunit:Interacts with heparin with a high affinity.,tissue specificity:Expressed in testis. Expressed exclusively at the connecting piece of the sperm., |
