WR4714 Western Blot analysis of HepG2 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LYPD3 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
$268.00–$328.00Price range: $268.00 through $328.00
Background:Predicted to enable laminin binding activity. Involved in negative regulation of smooth muscle cell apoptotic process. Located in extracellular space. [provided by Alliance of Genome Resources, Feb 2025]
Specifications
Size
50μL/100μL
Target
LYPD3
Reactivity
Human
Applications
WB, IHC, IP
MW(Calculated)
MW(Observed)
38kD
Host Species
Rabbit
Isotype
IgG,Kappa
Conjugate/Modification
Unmodified
Recommended Dilution Ratio
WB 1:1000-5000; IHC 1:100-300; IP 1:50-100 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification
Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality
Monoclonal
Clone Number
RM0801R
Immunogen
The specific immunogen used to produce this antibody is proprietary information.
Specificity
Endogenous
Gene Name
LYPD3; C4.4A; UNQ491/PRO1007
Protein Name
Ly6/PLAUR domain-containing protein 3; GPI-anchored metastasis-associated protein C4.4A homolog; Matrigel-induced gene C4 protein; MIG-C4
Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression is found in the tumor component of some noninvasive superficial lesions and in invasive and metastatic urothelial cancers. {ECO:0000269|PubMed:11179665, ECO:0000269|PubMed:12592373, ECO:0000269|PubMed:15012588}.
Signaling_pathway
Research Areas
Function
Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression. {ECO:0000269|PubMed:11179665, ECO:0000269|PubMed:11245483, ECO:0000269|PubMed:12592373, ECO:0000269|PubMed:15012588}.