SKU: WM0306 Categories: ,

LysRS Monoclonal Antibody

Price range: $268.00 through $328.00

SKU: WM0306-50
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Product details

Background:
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetLysRS
ReactivityHuman
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)68kD
MW(Observed)
Host SpeciesMouse
Isotype
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationAffinity purification
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone Number5H6
ImmunogenPurified recombinant fragment of LysRS(aa90-174) expressed in E. Coli.
SpecificityLysRS Monoclonal Antibody detects endogenous levels of LysRS protein.
Gene NameKARS
Protein NameLysyl-tRNA synthetase
Other NameKARS; / KIAA0070; / Lysine--tRNA ligase; / Lysyl-tRNA synthetase; / LysRS
SpeciesHuman
Gene ID3735
UniprotQ15046,
Species.1Mouse
Gene ID.1
Uniprot.1Q99MN1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization[Isoform Cytoplasmic]: Cytoplasm, cytosol . Cytoplasm . Nucleus . Cell membrane; Peripheral membrane protein . Secreted . Secretion is induced by TNF-alpha (PubMed:15851690). Cytosolic in quiescent mast cells. Translocates into the nucleus in response to mast cell activation by immunoglobulin E (PubMed:23159739). .; [Isoform Mitochondrial]: Mitochondrion .
Tissue ExpressionBone marrow,Brain,Cerebellum,Hepatoma,Placenta,
Signaling_pathway
Research Areas>>Aminoacyl-tRNA biosynthesis
FunctionCatalytic activity:ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).,similarity:Belongs to the class-II aminoacyl-tRNA synthetase family.,subunit:Homodimer (By similarity); also part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro.,
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