M-RIP Polyclonal Antibody
Ucallm_WP14346-50
Price range: $268.00 through $328.00
Product details
Background:
function:Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells., similarity:Contains 1 PH domain., similarity:Contains 2 PH domains., subcellular location:Colocalizes with F-actin., subunit:Binds F-actin through its N-terminus (By similarity). Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains.,
Specifications
| Size | 50μL/100μL |
| Target | M-RIP |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA, IHC |
| MW(Calculated) | |
| MW(Observed) | 115kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from the Internal region of human M-RIP. |
| Specificity | M-RIP Polyclonal Antibody detects endogenous levels of M-RIP protein. |
| Gene Name | MPRIP |
| Protein Name | Myosin phosphatase Rho-interacting protein |
| Other Name | MPRIP; / KIAA0864; / MRIP; / RHOIP3; / Myosin phosphatase Rho-interacting protein; / M-RIP; / Rho-interacting protein 3; / RIP3; / p116Rip |
| Species | Human |
| Gene ID | 23164;23164 |
| Uniprot | Q6WCQ1, |
| Species.1 | Mouse |
| Gene ID.1 | 26936 |
| Uniprot.1 | P97434, |
| Species.2 | Rat |
| Gene ID.2 | 116504 |
| Uniprot.2 | Q9ERE6 |
| Cellular Localization | Cytoplasm, cytoskeleton . Colocalizes with F-actin. |
| Tissue Expression | Aorta,Brain,Epithelium,Lymph,Melanoma,Muscle,Ovary,Tongue, |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.,similarity:Contains 1 PH domain.,similarity:Contains 2 PH domains.,subcellular location:Colocalizes with F-actin.,subunit:Binds F-actin through its N-terminus (By similarity). Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains., |
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