SKU: WP13043 Categories: ,

mAChR M1 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP13043-100
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Product details

Background:

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetmAChR M1
ReactivityHuman, Mouse, Rat
ApplicationsWB, ELISA
MW(Calculated)
MW(Observed)51kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; ELISA 1:5000; Not yet tested in other applications.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human CHRM1. AA range:295-344
SpecificitymAChR M1 Polyclonal Antibody detects endogenous levels of mAChR M1 protein.
Gene NameCHRM1
Protein NameMuscarinic acetylcholine receptor M1
Other NameCHRM1; / Muscarinic acetylcholine receptor M1
SpeciesHuman
Gene ID1128
UniprotP11229,
Species.1Mouse
Gene ID.112669
Uniprot.1P12657,
Species.2Rat
Gene ID.225229
Uniprot.2P08482
Cellular LocalizationCell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Tissue ExpressionAmygdala,Brain,
Signaling_pathwayCellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Nervous system >> Cholinergic synapse;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Research Areas>>Calcium signaling pathway; / >>cAMP signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>PI3K-Akt signaling pathway; / >>Cholinergic synapse; / >>Regulation of actin cytoskeleton; / >>Alzheimer disease; / >>Pathways of neurodegeneration - multiple diseases
FunctionFunction:The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.,similarity:Belongs to the G-protein coupled receptor 1 family.,subunit:Interacts with GPRASP2.,
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