Product details
Background:tissue specificity:Expression is restricted to melanoma and melanocyte cell lines and retina.,
U-Blot® MelanA Rabbit mAb
WR4241 Western blot analysis of lysates from Rat heart cells, using MYL2 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Specifications
| Size | 50μL/100μL |
| Target | Melan-A |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 13kD |
| MW(Observed) | 18kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0369R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | MLANA MART1 |
| Protein Name | Mart-1/Melan-A |
| Other Name | Melanoma antigen recognized by T-cells 1; / MART-1; / Antigen LB39-AA; / Antigen SK29-AA; / Protein Melan-A |
| Species | Human |
| Gene ID | 4633 |
| Uniprot | Q16655, |
| Species.1 | Mouse |
| Gene ID.1 | 17906 |
| Uniprot.1 | P51667 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Endoplasmic reticulum membrane |
| Tissue Expression | Highly expressed in type I muscle fibers. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Circulatory system >> Cardiac muscle contraction;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway |
| Research Areas | >>Cardiac muscle contraction; / >>Adrenergic signaling in cardiomyocytes; / >>Apelin signaling pathway; / >>Focal adhesion; / >>Tight junction; / >>Leukocyte transendothelial migration; / >>Regulation of actin cytoskeleton; / >>Shigellosis; / >>Salmonella infection; / >>Hypertrophic cardiomyopathy; / >>Dilated cardiomyopathy |
| Function | tissue specificity:Expression is restricted to melanoma and melanocyte cell lines and retina., |
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