Price range: $268.00 through $328.00
Background:
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | MIF |
| Reactivity | Human, Mouse, Rat |
| Applications | IF, WB, IHC, ELISA |
| MW(Calculated) | |
| MW(Observed) | 12kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IF 1:50-200; WB 1:500-2000; ELISA 1:10000-20000; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MIF. AA range:25-74 |
| Specificity | MIF Polyclonal Antibody detects endogenous levels of MIF protein. |
| Gene Name | MIF |
| Protein Name | Macrophage migration inhibitory factor |
| Other Name | MIF; / GLIF; / MMIF; / Macrophage migration inhibitory factor; / MIF; / Glycosylation-inhibiting factor; / GIF; / L-dopachrome isomerase; / L-dopachrome tautomerase; / Phenylpyruvate tautomerase |
| Species | Human |
| Gene ID | 4282 |
| Uniprot | P14174, |
| Species.1 | Mouse |
| Gene ID.1 | 17319 |
| Uniprot.1 | P34884, |
| Species.2 | Rat |
| Gene ID.2 | 81683 |
| Uniprot.2 | P30904 |
| Cellular Localization | Secreted . Cytoplasm . Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. . |
| Tissue Expression | Brain,Lens,Liver,Lung,Platelet,Skin |
| Signaling_pathway | |
| Research Areas | >>Tyrosine metabolism; / >>Phenylalanine metabolism; / >>Metabolic pathways |
| Function | Catalytic activity:Keto-phenylpyruvate = enol-phenylpyruvate.,Disease:Genetic variations in MIF are associated with susceptibility to systemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenile rheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despite medical treatment, many children still experience early joint destruction, necessitating surgical replacement.,Function:The expression of MIF at sites of inflammation suggest a role for the mediator in regulating the function of macrophage in host defense. Also acts as a phenylpyruvate tautomerase.,similarity:Belongs to the MIF family.,subunit:Homotrimer. Interacts with COPS5 and BNIPL., |
