MKK7 (Phospho Ser271/Thr275) rabbit pAb
Ucallm_WP10419-50
Price range: $288.00 through $368.00
Product details
Background:
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014],
Specifications
| Size | 50μL/100μL |
| Target | MKK7 Phospho Ser271/Thr275 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA, IHC |
| MW(Calculated) | |
| MW(Observed) | 47kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | phosphate |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized phosho peptide around human MKK7 (Ser271 and Thr275) |
| Specificity | This antibody detects endogenous levels of MKK7 only when phosphorylated at Ser271 or Thr275,and dually phosphorylated at two sites. |
| Gene Name | MAP2K7 JNKK2 MEK7 MKK7 PRKMK7 SKK4 |
| Protein Name | MKK7 (Ser271/Thr275) |
| Other Name | Dual specificity mitogen-activated protein kinase kinase 7; / MAP kinase kinase 7; / MAPKK 7; / JNK-activating kinase 2; / MAPK/ERK kinase 7; / MEK 7; / Stress-activated protein kinase kinase 4; / SAPK kinase 4; / SAPKK-4; / SAPKK4; / c-Jun N-terminal kinase kinase 2; / JNK kinase 2; / JNKK 2 |
| Species | Human |
| Gene ID | 5609 |
| Uniprot | O14733, |
| Species.1 | Mouse |
| Gene ID.1 | 26400 |
| Uniprot.1 | Q8CE90, |
| Species.2 | Rat |
| Gene ID.2 | 363855 |
| Uniprot.2 | Q4KSH7 |
| Cellular Localization | Nucleus. Cytoplasm . |
| Tissue Expression | Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway |
| Research Areas | >>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Protein processing in endoplasmic reticulum; / >>Osteoclast differentiation; / >>Tight junction; / >>Toll-like receptor signaling pathway; / >>T cell receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>TNF signaling pathway; / >>Neurotrophin signaling pathway; / >>GnRH signaling pathway; / >>Relaxin signaling pathway; / >>Alcoholic liver disease; / >>Alzheimer disease; / >>Huntington disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Salmonella infection; / >>Yersinia infection; / >>Hepatitis B; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Epstein-Barr virus infection; / >>Human immunodeficiency virus 1 infection; / >>Chemical carcinogenesis - reactive oxygen species; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation by specific MAP kinase kinase kinases such as MAP3K1/MEKK1, MAP3K3/MEKK3, MAP3K11/MLK3 and MAP3K12/DLK.,Function:Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3.,PTM:Activated by phosphorylation on Ser/Thr.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle., |
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