ML-IAP Polyclonal Antibody
Ucallm_WP13188-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jul 2013],
Specifications
| Size | 50μL/100μL |
| Target | ML-IAP |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| MW(Calculated) | |
| MW(Observed) | 21kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ML-IAP. AA range:162-211 |
| Specificity | ML-IAP Polyclonal Antibody detects endogenous levels of ML-IAP protein. |
| Gene Name | BIRC7 |
| Protein Name | Baculoviral IAP repeat-containing protein 7 |
| Other Name | BIRC7; / KIAP; / LIVIN; / MLIAP; / RNF50; / Baculoviral IAP repeat-containing protein 7; / Kidney inhibitor of apoptosis protein; / KIAP; / Livin; / Melanoma inhibitor of apoptosis protein; / ML-IAP; / RING finger protein 50 |
| Species | Human |
| Gene ID | 79444 |
| Uniprot | Q96CA5, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | A2AWP0 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm . Golgi apparatus . Nuclear, and in a filamentous pattern throughout the cytoplasm. Full-length livin is detected exclusively in the cytoplasm, whereas the truncated form (tLivin) is found in the peri-nuclear region with marked localization to the Golgi apparatus; the accumulation of tLivin in the nucleus shows positive correlation with the increase in apoptosis. |
| Tissue Expression | Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Apoptosis - multiple species;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Small cell lung cancer;Genetic Information Processing >> Folding, sorting and degradation >> Ubiquitin mediated proteolysis |
| Research Areas | >>Ubiquitin mediated proteolysis; / >>Apoptosis - multiple species; / >>Toxoplasmosis; / >>Pathways in cancer; / >>Small cell lung cancer |
| Function | Function:Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits caspase-3 and proteolytic activation of pro-caspase-9. Isoform 1 blocks staurosporine-induced apoptosis and isoform 2 blocks etoposide-induced apoptosis.,similarity:Belongs to the IAP family.,similarity:Contains 1 BIR repeat.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Nuclear, and in a filamentous pattern throughout the cytoplasm.,subunit:Binds to caspase-9. Interaction with SMAC via the BIR domain disrupts binding to caspase-9 and apoptotic suppressor activity. Interacts with TAB1. In vitro, interacts with caspase-3 and caspase-7 via its BIR domain.,tissue specificity:Very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines (isoform 1 and isoform 2). Isoform 2 (but not isoform 1) is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes., |
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