Name: U-Blot® MRE11 Rabbit mAb
SKU: WR4871
Price: 268 USD
Availability: InStock

Product details

Background:This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	MRE11
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	81kD
MW(Observed)	81kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1041R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	MRE11A HNGS1 MRE11
Protein Name	Double-strand break repair protein MRE11A
Other Name	MRE11A; / HNGS1; / MRE11; / Double-strand break repair protein MRE11A; / Meiotic recombination 11 homolog 1; / MRE11 homolog 1; / Meiotic recombination 11 homolog A; / MRE11 homolog A
Species	Human
Gene ID	4361
Uniprot	P49959,
Species.1	Mouse
Gene ID.1	17535
Uniprot.1	Q61216,
Species.2	Rat
Gene ID.2	64046
Uniprot.2	Q9JIM0
Cellular Localization	Nucleus . Chromosome, telomere . Chromosome . Localizes to discrete nuclear foci after treatment with genotoxic agents. .
Tissue Expression	Bladder,Brain,Epithelium,Lung,
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cellular senescence
Research Areas	>>Homologous recombination; / >>Non-homologous end-joining; / >>Cellular senescence
Function	cofactor:Manganese.,Disease:Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.,Disease:Defects in MRE11A may be a cause of breast cancer.,Function:Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.,miscellaneous:In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells.,online information:MRE11A mutation db,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MRE11/RAD32 family.,subcellular location:Localizes to discrete nuclear foci after treatment with genotoxic agents.,subunit:Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN (By similarity). Interacts with DCLRE1C/Artemis.,