MTDH Monoclonal Antibody
Ucallm_WM0328-50
Price range: $268.00 through $328.00
Product details
Background:
caution:Was originally (PubMed:15093543) thought to be a type II membrane protein but this is inconsistent with the results of multiple phosphorylation studies because this topology would locate the phosphorylation sites in the lumen or extracellularly rather than in the cytoplasm., function:Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance., induction:By TNF-alpha (at protein level). By HIV-1 infection of primary fetal astrocytes., miscellaneous:Knockdown significantly reduces the adhesion of cancer cells to lung microvascular endothelial cells and the reciprocal effect is observed following overexpression., subcellular location:In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus., subunit:Interacts with BCIPP, CREBBP/CBP and RELA/p65., tissue specificity:Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.,
Specifications
| Size | 50μL/100μL |
| Target | MTDH |
| Reactivity | Human |
| Applications | WB, IHC, IF, FC, ELISA |
| MW(Calculated) | 64kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 7E2 |
| Immunogen | Purified recombinant fragment of human MTDH expressed in E. Coli. |
| Specificity | MTDH Monoclonal Antibody detects endogenous levels of MTDH protein. |
| Gene Name | MTDH |
| Protein Name | Protein LYRIC |
| Other Name | MTDH; / AEG1; / LYRIC; / Protein LYRIC; / 3D3/LYRIC; / Astrocyte elevated gene-1 protein; / AEG-1; / Lysine-rich CEACAM1 co-isolated protein; / Metadherin; / Metastasis adhesion protein |
| Species | Human |
| Gene ID | 92140 |
| Uniprot | Q86UE4, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q80WJ7 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein . Cell junction, tight junction . Nucleus, nucleolus . Cytoplasm, perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus. . |
| Tissue Expression | Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme. |
| Signaling_pathway | |
| Research Areas | |
| Function | Caution:Was originally (PubMed:15093543) thought to be a type II membrane protein but this is inconsistent with the results of multiple phosphorylation studies because this topology would locate the phosphorylation sites in the lumen or extracellularly rather than in the cytoplasm.,Function:Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.,induction:By TNF-alpha (at protein level). By HIV-1 infection of primary fetal astrocytes.,miscellaneous:Knockdown significantly reduces the adhesion of cancer cells to lung microvascular endothelial cells and the reciprocal effect is observed following overexpression.,subcellular location:In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.,subunit:Interacts with BCIPP, CREBBP/CBP and RELA/p65.,tissue specificity:Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme., |
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