Name: U-Blot® Myeloperoxidase Rabbit mAb
SKU: WR4582
Price: 268 USD
Availability: InStock

U-Blot® Myeloperoxidase Rabbit mAb

HL-60 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4582 HL-60 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4582-50

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Product details

Background:Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]

Specifications

Size	50μL/100μL
Target	Myeloperoxidase
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	84kD
MW(Observed)	60,80-90kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:500-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0849R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	MPO
Protein Name	Myeloperoxidase(MPO)
Other Name
Species	Human
Gene ID	4353
Uniprot	P05164
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasmic
Tissue Expression	Leukemia,Leukocyte,Liver,Plasma,Saliva,
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Phagosome;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia
Research Areas	>>Drug metabolism - other enzymes; / >>Phagosome; / >>Neutrophil extracellular trap formation; / >>Transcriptional misregulation in cancer; / >>Acute myeloid leukemia
Function	Catalytic activity:Cl(-) + H(2)O(2) = HOCl + 2 H(2)O.,Catalytic activity:Donor + H(2)O(2) = oxidized donor + 2 H(2)O.,cofactor:Binds 1 calcium ion per heterodimer.,cofactor:Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer.,Disease:Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.,Function:Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.,online information:MPO mutation db,online information:Myeloperoxidase entry,similarity:Belongs to the peroxidase family. XPO subfamily.,subunit:Tetramer of two light chains and two heavy chains.,